Liver antibodies

Referred to Immunology, St Richard's Hospital, Chichester PO19 6SE.
 

Alternative names, keywords

Mitochondrial antibody, M2 antibody, smooth muscle antibody, liver-kidney-microsomal antibody, LKM antibody.

Samples required

Clotted blood (gold cap, 5 mL tube). Smaller tubes are available for paediatric samples.

Test indications

Investigation of possible autoimmune liver disease.

Test information

Indirect immunofluorescence test using liver-kidney-stomach substrate which reports the following:

Analyte   Information

ANA

Suitable only for investigation of liver disease, NOT for connective tissue disease. For CTD, please request ANA screen.

Where staining is observed, samples will automatically be tested for ANA by Hep2 immunofluorescence. Positive Hep2 staining (usually homogeneous) may be associated with autoimmune hepatitis.

Smooth muscle antibody

Positive results are associated with autoimmune, viral and drug-induced hepatitis.

Liver-kidney-microsomal antibody

Positive results are associated with autoimmune hepatitis.

Mitochondrial antibody

Positive staining on this test needs to be confirmed using a second-line test – results will follow.

M2 antibody

Performed by immunoassay; positive results are associated with primary biliary cholangitis.

Method: Indirect immunofluorescence with Hep2 substrate.

M2 antibody: Phadia 250 ELIA

Reference range

Analyte

Report

ANA

Negative, or cascaded to ANA by Hep2 IIF method.

Smooth muscle antibody

Negative, weak positive or positive.

Liver-kidney-microsomal antibody

Negative, weak positive or positive.

Mitochondrial antibody

Negative, weak positive or positive.

M2 antibody

Negative, equivocal or positive.

Turnaround time

14 days.

Enquiries

Immunology (Referrals)