1,25-dihydroxyvitamin D

Referred to Berkshire & Surrey Pathology Services, Frimley Park Hospital NHS Foundation Trust, Portsmouth Road, Frimley, Surrey GU16 7UJ

Alternative names, keywords

1,25-dihydroxycholecalciferol, 1,25-OHD, 1,25-dihydroxyvitamin D3

Samples required

Clotted blood (gold cap, 5 mL tube). Protect from light, deliver immediately to lab, on ice, plus

Clotted blood (gold cap, 5 mL tube) for simultaneous bone profile. Smaller tubes are available for paediatric samples.

Do not send samples on ice via pneumatic tube (pod) system.

Test indications

Very rarely indicated - do not request for routine suspected vitamin D deficiency or toxicity.

Monitoring of 1,25-D may occasionally be useful in patients treated with calcitriol (1,25D) or alfacalcidol (1α-hydroxycholecalciferol), and in cases where abnormal vitamin D metabolism is suspected, e.g. inherited rickets, hypercalcaemic sarcoidosis.

Reference range

48 - 192 pmol/L

Turnaround time

Referral lab quotes turnaround time of 10 working days, however additional time must be allowed for packing, dispatch and delivery of sample, and return and processing of results.


Biochemistry (Referrals)