Alternative names, keywords

Aspartate aminotransferase, SGOT, glutamic oxaloacetic transaminase

Samples required

Clotted blood (gold cap, 5 mL tube). Smaller tubes are available for paediatric samples.

Avoid haemolysis.

Test indications


Factors affecting test performance/ results

Avoid haemolysis. Contamination with erythrocyte contents will  elevate results, because the ALT activity in erythrocytes is higher than in normal serum. The degree of interference may be variable depending on the content of analyte in the lysed erythrocytes.

Cyanokit (hydroxocobalamin) may cause interference with results.

Physiological plasma concentrations of sulfasalazine and sulfapyridine may lead to false results.

In very rare cases, gammopathy, in particular type IgM (Waldenström’s macroglobulinemia), may cause unreliable results.

Results should always be assessed in conjunction with the patient’s medical history, clinical examination and other findings.

Reference range

male: 0 - 40 IU/L
female: 0 - 32 IU/L
Source: Roche Cobas® AST kit insert, 07-2013, V10.0

Turnaround time

Same day (within 2h if urgent)


Biochemistry (Automation)