Viral hepatitis - diagnostic virology

Consider for jaundice, deranged liver enzymes (ALT/AST).

Samples Test Pathogens covered Comment

5 mL clotted blood.

Smaller tubes are available for paediatric samples.

   

1st line testing:

HAV IgM
HBsAg
Anti-HCV*

Hepatitis A virus (HAV)

Hepatitis B virus (HBV)

Hepatitis C virus (HCV)

*If acute HCV is suspected, then HCV core antigen or HCV PCR is also needed.

If 1st line testing is negative & other symptoms suggest CMV/EBV:

CMV IgM
EBV VCA IgM

Cytomegalovirus (CMV)

Epstein-Barr virus (EBV)

 

In addition to the 1st line screen above, if there is travel history to HEV endemic countries or ALT >1000 IU/L:

HEV (IgM & IgG)**

Hepatitis E virus (HEV)

**HEV serology is referred to Colindale.

Virus Reference Department, Microbiology Services Colindale, 61 Colindale Avenue, London NW9 5HT

If patient is immunosuppressed:

HSV DNA
RealTime ACE triplex PCR

Herpes simplex virus (HSV)

CMV/EBV/adenovirus

 

 

If patient is neonate:

HSV DNA RealTime,
Enterovirus RNA
Parechovirus RNA
CMV DNA
 

Herpes simplex virus (HSV)

Enterovirus & Parechovirus

CMV

 

Turnaround time

see virology and microbiological serology turnaround times.

** HEV serology: referral lab quotes target turnaround time of 8 days, however additional time must be allowed for packing, dispatch and delivery of sample, and return and processing of results.

Enquiries

Microbiology (Virology and Serology), ext. 64627